Membrane protein production inEscherichia colicell-free lysates
نویسندگان
چکیده
منابع مشابه
Cell-free protein synthesis in lysates of Drosophila melanogaster cells.
A procedure is described for preparing cell-free protein synthesizing lysates from Drosophila melanogaster tissue culture cells and embryos. Preparation of translationally active lysates from tissue culture cells is dependent on the presence of rat liver supernatant during cell lysis to inhibit ribonuclease activity. After micrococcal nuclease treatment of the lysate, protein synthesis is depen...
متن کاملPartial acid hydro lysates of proteins; assay of liquid protein hydro lysates in protein-depleted rats.
Experiments to determine the comparative nutritive value of partial acid hydrolysates of various proteins have been carried out for several years in this laboratory. Most of this work has involved studies of nitrogen balance in dogs wherein the hydrolysates were administered intravenously. Short term experiments, i.e. 4 to 5 days, were used to determine minimum nitrogen levels at wb.ich the N b...
متن کاملProtein synthesis in cell-free reticulocyte lysates on multi-hour incubation.
Cell-free protein synthesis in rabbit reticulocyte lysate translation mixtures has been studied during multi-hour incubations. In an impaired lysate obtained from cells stored at 0 degrees C before lysis, and showing a low initial rate of synthesis, translation could be stimulated by a factor of 4 by including RNase inhibitor and additional ATP and GTP. In translation mixtures prepared from nor...
متن کاملEnhanced membrane protein expression by engineering increased intracellular membrane production
BACKGROUND Membrane protein research is frequently hampered by the low natural abundance of these proteins in cells and typically relies on recombinant gene expression. Different expression systems, like mammalian cells, insect cells, bacteria and yeast are being used, but very few research efforts have been directed towards specific host cell customization for enhanced expression of membrane p...
متن کاملTuning microbial hosts for membrane protein production
The last four years have brought exciting progress in membrane protein research. Finally those many efforts that have been put into expression of eukaryotic membrane proteins are coming to fruition and enable to solve an ever-growing number of high resolution structures. In the past, many skilful optimization steps were required to achieve sufficient expression of functional membrane proteins. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2015
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2015.04.045